ABSTRACT Terpenes are secondary metabolites produced from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). Considering their role against biotic and abiotic stress conditions, the D-limonene, a terpene recognized for its roles in plant defense and as pigment molecules, was tested to monitor its impact in the accumulation of carotenoid terpene pigments during tomato fruit ripening. The concentrations of 0.0, 1.0, and 2.0 mL·L–1 were sprayed weekly on tomato plants ‘Micro-Tom’ until the harvest. The fruits were harvested at the mature green stage (MG), the breaker stages (BR), three days after the breaker (BR + 3), the red ripe stage (RR), and the concentration of carotenoids was evaluated. The application of D-limonene enhanced the concentration of carotenoids in the early stages of tomato fruit ripening and ripe fruits, and the accumulation of carotenoids was greater following dose increment. This result can bring important contributions to horticulture and food nutrition by linking plant defense and the accumulation of molecules with recognized antioxidant capacity in fruit.

Garlic has great economic and social importance for the region of Curitibanos (SC). However, a number of factors has hindered production. One of these factors is the high cost of fertilizers, especially the nitrogenous ones, an element of which the crop is highly demanding. To reduce the impacts, the use of PGPR (Plant Growth Promoting Rhizobacteria), especially fluorescent Pseudomonas to induce plant development and their production has been widely studied. However, little is known about these microorganisms and garlic. Fluorescent Pseudomonas were isolated from rhizosphere of garlic cultivated in soil of the Curitibanos region (SC). Twenty-three isolates were phenotypical and genotypically analyzed. The phosphate solubiliziation, IAA (Indole Acetic Acid) production and Biological Nitrogen Fixation (BNF) in vitro, besides the effect on plant under controlled conditions were evaluated. The phenotypic characteristics matched to those described for the group of fluorescent Pseudomonas. Fifty two percent of isolates solubilized Ca3(PO4)2, 69% presented FBN and 72% produced IAA. Genetically, all the isolates belonged to the genus Pseudomonas and resembled to Pseudomonas kribbensis, P. azotoformans or P. baetica. The strain CBSAL02 induced significant increase at fourth leaf size, having high correlation to the bulbification, suggesting its potential as a growth promoter and, consequently, production.

El ajo tiene una gran importancia económica y social para la región de Curitibanos (SC). Sin embargo, una serie de factores ha impedido la producción. Uno de estos factores es el alto costo de los fertilizantes, especialmente los nitrogenados, un elemento por el cual el cultivo es muy exigente. Para reducir los impactos, se ha estudiado ampliamente el uso de RPCP (Rizobacterias promotoras del crecimiento de las plantas), especialmente Pseudomonas fluorescentes para inducir el desarrollo de las plantas y su producción. Sin embargo, poco se sabe sobre estos microorganismos y el ajo. Las Pseudomonas fluorescentes se aislaron de la rizosfera de ajo cultivado en el suelo de la región de Curitibanos (SC). Veintitrés aislados fueron fenotípicos y genotípicamente analizados. Se evaluaron la solubilización de fosfato, la producción de AAI (ácido acético indol) y la fijación biológica de nitrógeno (FBN) in vitro, además del efecto en la planta bajo condiciones controladas. Las características fenotípicas coinciden con las descritas para el grupo de Pseudomonas fluorescentes. Cincuenta y dos por ciento de los aislados solubilizaron Ca3(PO4)2, 69% presentaron FBN y 72% produjeron AAI. Genéticamente, todos los aislados pertenecían al género Pseudomonas y se parecían a Pseudomonas kribbensis, P. azotoformans o P. baetica. La cepa CBSAL02 indujo un aumento significativo en el tamaño de la cuarta hoja, teniendo una alta correlación con la bulbificación, lo que sugiere su potencial como promotor del crecimiento y, en consecuencia, la producción.

Resumo Contexto A cirurgia bariátrica é considerada a melhor opção para o tratamento da obesidade, cujos pacientes são considerados de alto risco para fenômenos tromboembólicos. Objetivos Comparar o uso de doses diferentes de heparina de baixo peso molecular (HBPM) na profilaxia da trombose venosa profunda (TVP) em pacientes candidatos à cirurgia bariátrica em relação ao risco de TVP, alteração na dosagem do fator anti-Xa e sangramento pré ou pós-operatório. Métodos Estudo comparativo transversal em pacientes submetidos à cirurgia bariátrica distribuídos em dois grupos, que receberam doses de HBPM de 40 mg (grupo controle, GC) e 80 mg (grupo de estudo, GE). Foram avaliados por ultrassonografia vascular e dosagem de KPTT, TAP, plaquetas e fator anti-Xa. Resultados Foram avaliados 60 pacientes, sendo 34 no GC e 26 no GE. Foi observada diferença significativa somente no peso (p = 0,003) e índice de massa corporal (p = 0,018) no GE em relação ao GC. Não houve diferença na dosagem de KPTT, TAP, plaquetas e fator anti-Xa entre os grupos. Não foram detectados TVP ou sangramentos significativos em ambos os grupos. Conclusões Não houve diferença estatisticamente significativa na utilização de doses maiores de HBPM na profilaxia da TVP em pacientes candidatos à cirurgia bariátrica em relação ao risco de TVP, dosagem do fator anti-Xa e sangramento pré ou pós-operatório.

Abstract Background Bariatric surgery is considered the best treatment option for patients with obesity who are classed as high risk for thromboembolic events. Objectives To compare two different doses of low weight molecular heparin (LWMH) for prevention of deep venous thrombosis (DVT) in candidates for bariatric surgery, in terms of DVT risk, abnormal anti-Xa levels, and preoperative and/or postoperative bleeding. Methods A cross-sectional comparative study of bariatric surgery patients divided into two groups given different doses of LWMH; 40 mg of LWMH (control group, CG) and 80 mg of LWMH (study group, SG), both evaluated by vascular ultrasonography (VU) and according to the results of PTT, PT, platelets, and anti-Xa factor assays. Results Sixty patients were evaluated, 34 in the CG and 26 in the SG. The only significant differences between the patients in the SG and the CG were weight (p = 0.003) and body mass index (p = 0.018). There were no differences between the groups in PTT, PT, platelets, or anti-Xa factor levels. There was no DVT or significant bleeding in either group. Conclusions There were no statistical differences when higher doses of LWMH were used for prevention of DVT in bariatric surgery patients, in terms of DVT risk, anti-Xa factor levels, or preoperative and postoperative bleeding.